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Orbilia look-alike with minute brown tufts
Edvin Johannesen,
30-12-2018 17:11
These small apothecia are growing on deciduous wood, possibly Salix caprea. In the microscope I see small brown tufts of bottle-shaped cells. Photos of these cells are in KoH.
Any suggestion?
Edvin Johannesen,
30-12-2018 17:14
Re : Orbilia look-alike with minute brown tufts
Spores are allantoid. Here mounted in Congo Red in water. (1000x oil)
Edvin Johannesen,
30-12-2018 17:16
Re : Orbilia look-alike with minute brown tufts
Ascus pores negative with Lugol, but there may be a slight colouring of the extreme ascus pore in some asci. I also wonder what the rounded cells on top of the asci are. An epithecium?
Lothar Krieglsteiner,
30-12-2018 17:46
Re : Orbilia look-alike with minute brown tufts
Hello Edvin,
perhaps a Calycina with a Chalara-anamorph?
Best regards, Lothar
Hans-Otto Baral,
30-12-2018 18:14
Re : Orbilia look-alike with minute brown tufts
Yes, and I think it is Calycina vulgaris if the iodine reaction is actually negative.
The cells on the hymenium are from another organism. Algae?
The cells on the hymenium are from another organism. Algae?
Edvin Johannesen,
30-12-2018 18:16
Re : Orbilia look-alike with minute brown tufts
Thanks for the suggestion, Lothar. I didn't think of the possibilitiy of the brown "hairs" being an anamorph. Calycina is currently in Hyaloscyphaceae, right? I see no hairs on the margin. Is there a Calycina with such spores? Does anyone have a current key to Calycina?
Regards.
Hans-Otto Baral,
30-12-2018 18:18
Re : Orbilia look-alike with minute brown tufts
Placement is in Pezizellaceae now. Pezizella is a synonym of Calycina, and its type species P. sordida is a syonym of C. vulgaris.
For a key it is too premature, I am unaware, and only know my personal key.
For a key it is too premature, I am unaware, and only know my personal key.
Edvin Johannesen,
30-12-2018 18:20
Re : Orbilia look-alike with minute brown tufts
Zotto, I don't think the rounded cells are algae or from another organism. In any case, I think you are right about C. vulgaris. The Chalara anamorph is depicted here: http://asco-sonneberg.de/pages/gallery/calycina-vulgaris-130106-zj-mcol-02jj33291.php?group_id=33241&position=20
Thanks a lot, guys! :-)
Hans-Otto Baral,
30-12-2018 18:23
Re : Orbilia look-alike with minute brown tufts
Yes for the anamorph.
These strange cells have a gel sheath if I see this right. I would prefer seeing them in an unstained water mount to better make up my mind. But from the fungus I cannot imagine. Chalara conidia are rectangular.
These strange cells have a gel sheath if I see this right. I would prefer seeing them in an unstained water mount to better make up my mind. But from the fungus I cannot imagine. Chalara conidia are rectangular.
Edvin Johannesen,
30-12-2018 18:50
Re : Orbilia look-alike with minute brown tufts
You are right about the gel. When I first saw the rounded cells in water, they were indeed embedded in a gel. I made a new water mount now, from a different apothecium, but the cells weren't there. So I guess they must have been "alien". Thanks again.