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Hello, Found by Laurens van der Linde, April 2024
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Bonjour,Trouvé sous paille humide, autour d'un je
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Bernard CLESSE
Bonsoir à toutes et tous,Pourriez-vous m'aider à
Fungus on Cow Dung
John Leach,
02-12-2017 16:00
I recently collected and incubated some cow dung from Longshaw in Derbyshire. Several common species were present and identified but I am having difficulty in putting a name to this one. My original thoughts were of a Coprotus but now have doubts.
Fruitbody 0.5 - 1 mm
Spore size = 17.2 - 20 x 9.8 - 11 micron
Ascus = 190 - 210 x 24 micron
Mz = Negative
Any help as always much appreciated
Best Wishes - John
Nicolas VAN VOOREN,
02-12-2017 16:09
Re : Fungus on Cow Dung
The wall of asci is strongly MLZ+ and the ascospores seem to be verrucose. You should search in the Iodophanus or Thecotheus genera.
Malcolm Greaves,
02-12-2017 16:14
Re : Fungus on Cow Dung
John
With the granular spores try Thecotheus.
Mal
Beat me to it Nicolas
With the granular spores try Thecotheus.
Mal
Beat me to it Nicolas
John Leach,
02-12-2017 16:19
Re : Fungus on Cow Dung
Thanks Nicolas.
I had considered Iodophanus. Will re check and look at Thecotheus too
I had considered Iodophanus. Will re check and look at Thecotheus too
John Leach,
02-12-2017 16:20
Re : Fungus on Cow Dung
Thanks Mal
Jacky Launoy,
02-12-2017 16:51
Re : Fungus on Cow Dung
I thinck it is Iodophanus carneus
Michel Delpont,
02-12-2017 17:12
Re : Fungus on Cow Dung
Corresponds well to I.carneus. Taxon variable especially by the size of the spores.
Michel.
Michel.
Malcolm Greaves,
02-12-2017 17:21
Re : Fungus on Cow Dung
John
You say Meltzers negative but have a couple of photos with strong blue cast was this another stain? Iodophanus stains very strongly in Meltzers.
Mal
You say Meltzers negative but have a couple of photos with strong blue cast was this another stain? Iodophanus stains very strongly in Meltzers.
Mal
John Leach,
02-12-2017 19:10
Re : Fungus on Cow Dung
Thanks everyone. I had ruled out Iodophanus carneus on the size of spores and the swollen flask ends to most paraphyses. It certainly is quite variable!
I can only put down my comment on a negative reaction to Melzers as another senior moment ;-)
I can only put down my comment on a negative reaction to Melzers as another senior moment ;-)
Hans-Otto Baral,
02-12-2017 20:53
Re : Fungus on Cow Dung
Dear John
I agree with Iodophanus carneus. But:
I don't think the species is variable, at least I know it rather constant.
If you have the fungus stil fresh, I beg you to make a mount in tap water without any ingredients:
- The paraphyses should be multiguttulate (strikingly, but don't press on the slide).
- The asci of this species are somewhat hemiamyloid, which means that Melzer's may be rather negative but Lugol positive, and only when you put it first in KOH, then Melzer gives also a strong bvlue reaction.
Of course, your emptied asci are clearly blue, only those which contain spores are not visibly amyloid.
Zotto
I agree with Iodophanus carneus. But:
I don't think the species is variable, at least I know it rather constant.
If you have the fungus stil fresh, I beg you to make a mount in tap water without any ingredients:
- The paraphyses should be multiguttulate (strikingly, but don't press on the slide).
- The asci of this species are somewhat hemiamyloid, which means that Melzer's may be rather negative but Lugol positive, and only when you put it first in KOH, then Melzer gives also a strong bvlue reaction.
Of course, your emptied asci are clearly blue, only those which contain spores are not visibly amyloid.
Zotto
John Leach,
02-12-2017 21:47
Re : Fungus on Cow Dung
Hello my good friend Zotto
I still have fresh material and will do a tap water mount as you suggest
Best Wishes - John
I still have fresh material and will do a tap water mount as you suggest
Best Wishes - John
John Leach,
02-12-2017 23:22
Re : Fungus on Cow Dung
Mounted in Lugol and showing bifurcate paraphyses
Peter Püwert,
02-12-2017 23:44
Re : Fungus on Cow Dung
Hi, after my observations microscopic characteristic features can already diverge according to stage of development. In colour the species also has a proper variety.
Greetings Peter.
Greetings Peter.
Hans-Otto Baral,
02-12-2017 23:44
Re : Fungus on Cow Dung
Thanks John, I remember our stay in Wentworth Castle!
Splendid, the ascus is fully turgescent and I see the guttules in the paraphyses, though not many. The iodine reaction is not spectacular, but it is there.
Zotto
Splendid, the ascus is fully turgescent and I see the guttules in the paraphyses, though not many. The iodine reaction is not spectacular, but it is there.
Zotto
John Leach,
03-12-2017 00:04
Re : Fungus on Cow Dung
Thank you Peter. Appreciated!
Yes, it was an excellent week at Wentworth Castle Zotto. I learnt so much from so many!
Yes, it was an excellent week at Wentworth Castle Zotto. I learnt so much from so many!