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28-06-2025 16:00

Josep Torres Josep Torres

Hello.A tiny fungus shaped like globose black grai

01-07-2025 23:37

Josep Torres Josep Torres

Hello.A Pleosporal symbiotic organism located and

02-07-2025 17:26

Yanick BOULANGER

BonjourRécolté sur une brindille au fond d'un fo

03-07-2025 20:08

Francois Guay Francois Guay

I found this interesting yellowish asco growing on

03-07-2025 18:40

Castillo Joseba Castillo Joseba

me mandas el material seco de Galicia (España) re

02-07-2025 18:45

Elisabeth Stöckli

Bonsoir,Sur feuilles d'Osmunda regalis (Saulaie),

02-07-2025 09:32

Nicolas VAN VOOREN Nicolas VAN VOOREN

Hello, bonjour.Here is the paper I'm searching for

30-06-2025 16:56

Lydia Koelmans

Please can anyone tell me the species name of the

30-06-2025 12:09

Edvin Johannesen Edvin Johannesen

This tiny, rather "rough" erumpent asco was found

30-06-2025 06:57

Ethan Crenson

Hi all, Another find by a friend yesterday in Bro

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Mould which easily break when mounted in water or Stains
Stephen Martin, 02-12-2017 08:36
Stephen MartinHi, I got this mould as a contaminant while studying another microfungus inoculated from a decaying log. On various media (PDA, SDA, Czapek) it forms an olive green growth  (3cm / 14C / 7 days) with a yellowish tinge. First indication is that of a Penicillium sp. but under the microscope I could not make heads and tails. The prime character I can describe is that the phialides and hypha of the conidiogenesis apparatus breaksing up into  constituent pieces giving this polymorphic observation of spores, hyphae and septate(?) phialides of various shapes and sizes. These septate hyphae have tiny projections indicating that they used to bear spores and hence part of the fruiting organ.

Rarely I see spores in short chains but often they are very hygrophobic and entrapped in tiny bubbles hindering the view of the conidiaphore+apparatus. Is there a specific mould with this character - breaking out easily? I strongly doubt it is a  Penicillium as I managed to see a fruiting part with budding spore.
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Angel Pintos, 02-12-2017 09:32
Angel Pintos
Re : Mould which easily break when mounted in water or Stains
Looks like Cladosporium sp.

regards

Angel
Joey JTan, 02-12-2017 17:03
Re : Mould which easily break when mounted in water or Stains
Yes this is a Cladosporium sp. The conidial chains easily fragment and can be seen best by making a tape mount. Gently press a small piece of transparent tape against the colony and mount it in water (place it on a drop of water, don't just stick it to the slide itself). You should see the chains still connected. The larger, 2-celled, shield-like ramoconidia are indicative of Cladosporium and give rise to branching conidia chains.
Stephen Martin, 07-12-2017 23:29
Stephen Martin
Re : Mould which easily break when mounted in water or Stains
Hi thank you for your replies. It took time to come back but I did not forget you. The tape method did a lovely job. I cut a small squarish piece of tape using tweezers and clean scissors, laid the sticky surface on the outer part of the colony, gave a very gentle press (like a touch rather than a press), placed the sticky surface of a drop of water on a mounting slide and mounted under a microscope. It was interesting to observe that this species had clamp junctions. 

Now, I could see the conidiogenesis structure very well and I drew a quick illustration :-) 

I don't think I dare to follow the Cladosporium key of a meticulous monograph to reach species level!
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