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19-07-2018 00:11

Thorben Hülsewig

Hi there,yesterday i found on ground this ascomyce

21-07-2018 11:02

Valencia Lopez Francisco Javier

Hola a todos/asTengo estas Escutellinia recolectad

20-07-2018 07:04

Blasco Rafael Blasco Rafael

Hola puede ser la Orbilia que priopongo ??sonre ma

20-07-2018 22:52

Viktorie Halasu Viktorie Halasu

Hello forum,here's another Peziza close to exogela

13-07-2018 17:59

Viktorie Halasu Viktorie Halasu

Hello forum,we found this small Peziza (5-12 mm di

09-07-2018 20:27

Andgelo Mombert

Bonsoir à tous, Sur tige morte de Adenostyles sp

17-07-2018 21:24

BERNARD CLESSE BERNARD CLESSE

Voici un asco blanc récolté dans un fossé tourb

13-07-2018 19:59

Andgelo Mombert

Bonsoir,Sous Picea, Alnus, Sorbus, vers 1500 m d'a

18-07-2018 18:33

Guillaume Eyssartier

Bonjour à tous, je vous envoie cet étrange asco

19-07-2018 13:29

Savic Dragisa

I think this is Peziza tenacella, the ornamentatio

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Mould which easily break when mounted in water or Stains
Stephen Mifsud, 02-12-2017 08:36
Stephen MifsudHi, I got this mould as a contaminant while studying another microfungus inoculated from a decaying log. On various media (PDA, SDA, Czapek) it forms an olive green growth  (3cm / 14C / 7 days) with a yellowish tinge. First indication is that of a Penicillium sp. but under the microscope I could not make heads and tails. The prime character I can describe is that the phialides and hypha of the conidiogenesis apparatus breaksing up into  constituent pieces giving this polymorphic observation of spores, hyphae and septate(?) phialides of various shapes and sizes. These septate hyphae have tiny projections indicating that they used to bear spores and hence part of the fruiting organ.

Rarely I see spores in short chains but often they are very hygrophobic and entrapped in tiny bubbles hindering the view of the conidiaphore+apparatus. Is there a specific mould with this character - breaking out easily? I strongly doubt it is a  Penicillium as I managed to see a fruiting part with budding spore.
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Pintos Angel, 02-12-2017 09:32
Pintos Angel
Re : Mould which easily break when mounted in water or Stains
Looks like Cladosporium sp.

regards

Angel
Joey JTan, 02-12-2017 17:03
Re : Mould which easily break when mounted in water or Stains
Yes this is a Cladosporium sp. The conidial chains easily fragment and can be seen best by making a tape mount. Gently press a small piece of transparent tape against the colony and mount it in water (place it on a drop of water, don't just stick it to the slide itself). You should see the chains still connected. The larger, 2-celled, shield-like ramoconidia are indicative of Cladosporium and give rise to branching conidia chains.
Stephen Mifsud, 07-12-2017 23:29
Stephen Mifsud
Re : Mould which easily break when mounted in water or Stains
Hi thank you for your replies. It took time to come back but I did not forget you. The tape method did a lovely job. I cut a small squarish piece of tape using tweezers and clean scissors, laid the sticky surface on the outer part of the colony, gave a very gentle press (like a touch rather than a press), placed the sticky surface of a drop of water on a mounting slide and mounted under a microscope. It was interesting to observe that this species had clamp junctions. 

Now, I could see the conidiogenesis structure very well and I drew a quick illustration :-) 

I don't think I dare to follow the Cladosporium key of a meticulous monograph to reach species level!
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