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18-10-2018 23:23

Johan Myhrer

Have been struggeling with these for a while, foun

18-10-2018 23:55

Yaroslav Mieshkov Yaroslav Mieshkov

Hello,Does anyone has the following paper? Bertau

08-10-2018 14:51

Edvin Johannesen Edvin Johannesen

I came across these dense mats of apothecia seated

18-10-2018 10:37

Bernard Declercq Bernard Declercq

Hello, Can anyone supply me following paper? Pa

17-10-2018 07:10

Ethan Crenson

In Northeastern North America.  On the banks of a

15-10-2018 18:55

hannie wijers

Hello, I'd like some help if possibleIn Beilen I

16-10-2018 23:33

Valencia Lopez Francisco Javier

Hola a todos/asPeziza recolectada en el sur de Esp

03-10-2018 22:13

François Bartholomeeusen

Good evening forum members,Can this be Hyalorbilia

14-10-2018 19:25

Marja Pennanen

Hi, on a Populus leaf among other things, I found

15-10-2018 10:52

Castillo Joseba Castillo Joseba

Del pasado sabado en bosque de avellanos,   pare

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Mould which easily break when mounted in water or Stains
Stephen Mifsud, 02-12-2017 08:36
Stephen MifsudHi, I got this mould as a contaminant while studying another microfungus inoculated from a decaying log. On various media (PDA, SDA, Czapek) it forms an olive green growth  (3cm / 14C / 7 days) with a yellowish tinge. First indication is that of a Penicillium sp. but under the microscope I could not make heads and tails. The prime character I can describe is that the phialides and hypha of the conidiogenesis apparatus breaksing up into  constituent pieces giving this polymorphic observation of spores, hyphae and septate(?) phialides of various shapes and sizes. These septate hyphae have tiny projections indicating that they used to bear spores and hence part of the fruiting organ.

Rarely I see spores in short chains but often they are very hygrophobic and entrapped in tiny bubbles hindering the view of the conidiaphore+apparatus. Is there a specific mould with this character - breaking out easily? I strongly doubt it is a  Penicillium as I managed to see a fruiting part with budding spore.
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Pintos Angel, 02-12-2017 09:32
Pintos Angel
Re : Mould which easily break when mounted in water or Stains
Looks like Cladosporium sp.

regards

Angel
Joey JTan, 02-12-2017 17:03
Re : Mould which easily break when mounted in water or Stains
Yes this is a Cladosporium sp. The conidial chains easily fragment and can be seen best by making a tape mount. Gently press a small piece of transparent tape against the colony and mount it in water (place it on a drop of water, don't just stick it to the slide itself). You should see the chains still connected. The larger, 2-celled, shield-like ramoconidia are indicative of Cladosporium and give rise to branching conidia chains.
Stephen Mifsud, 07-12-2017 23:29
Stephen Mifsud
Re : Mould which easily break when mounted in water or Stains
Hi thank you for your replies. It took time to come back but I did not forget you. The tape method did a lovely job. I cut a small squarish piece of tape using tweezers and clean scissors, laid the sticky surface on the outer part of the colony, gave a very gentle press (like a touch rather than a press), placed the sticky surface of a drop of water on a mounting slide and mounted under a microscope. It was interesting to observe that this species had clamp junctions. 

Now, I could see the conidiogenesis structure very well and I drew a quick illustration :-) 

I don't think I dare to follow the Cladosporium key of a meticulous monograph to reach species level!
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