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21-03-2026 22:59

Petr Soucek

Good evening, I would appreciate some advice on th

21-03-2026 15:13

Lepista Zacarias

Hello everyone, Does any one know of any literatu

20-03-2026 12:53

Stefan Blaser

Hello everybody, In the field, from distance, my

20-10-2017 09:23

Garcia Susana

Este otro crecía en el mismo trocito de madera qu

20-03-2026 16:16

Edvin Johannesen Edvin Johannesen

These 0.5 mm diam. acervuli were breaking through

19-03-2026 19:34

Filip Fuljer Filip Fuljer

Hello everyone,a few days ago I collected this str

19-03-2026 18:25

William Slosse William Slosse

Good evening everyone, On 18/03/26 I found a few

17-03-2026 10:09

François Freléchoux François Freléchoux

Bonjour, Voici la description rapide d'un petit d

19-03-2026 15:58

Stefan Blaser

Hello everybody, I hope for some hints... Macro:

19-03-2026 17:50

Enrique Rubio Enrique Rubio

Hi to everybodyThese thiny, blackish pseudothecia

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Microglossum
Malcolm Greaves, 13-10-2017 11:28
Malcolm  GreavesI would have recorded these as Microglossum olivaceum but having seen the article in Mycologia it now looks as though it should now be refered to as M tenebrosum.
Found on a thin layer of soil in an abandoned chalk quarry. Spores were 13-14.3 x 4
Am I right in thinking this?
Thanks
Mal
  • message #50704
  • message #50704
  • message #50704
Hans-Otto Baral, 14-10-2017 11:55
Hans-Otto Baral
Re : Microglossum
Dear Malcolm
for me it looks actually like M. tenebrosum as figured by Kucera et al. 2017. I have also photos of a sample from northern Germany in my tenebrosum folder (under the name M. nudipes) that matches perfectly by macroscopy.
Zotto
Malcolm Greaves, 14-10-2017 22:04
Malcolm  Greaves
Re : Microglossum
Thanks Zotto
Malcolm Greaves, 25-11-2017 18:38
Malcolm  Greaves
Re : Microglossum
Zotto
This sample has been found to be a DNA match for tenebrosum.
Mal
Hans-Otto Baral, 25-11-2017 18:53
Hans-Otto Baral
Re : Microglossum
Thanks, Malcolm! I see that in GenBank several sequences exist, moist being 100% identical. Do you have the ITS sequence and did you test a BLAST with it?
Zotto
Malcolm Greaves, 25-11-2017 22:23
Malcolm  Greaves
Re : Microglossum
Zotto
The project concerning Microglossums in Wales is being run by another group. It was them who did/organised the DNA test on my sample. I was told that "the result gave a barcode sequence which is consistant with that published for the type specimen" but I have no further details. If it would help I am sure I could find out more information.
Mal
Hans-Otto Baral, 26-11-2017 09:05
Hans-Otto Baral
Re : Microglossum
Thanks, this is enough. The type is NR_148113, and it is 100% identical with some other sequences in the ITS region.

Only one remark: Is it the ITS1-5.8S-ITS2 region that was sequenced (briefly called ITS region)?
Zotto
Malcolm Greaves, 26-11-2017 12:23
Malcolm  Greaves
Re : Microglossum
David who is coordinating the projects has said yes they are using part of the ITS region and each sequence is then run through BLAST to compare with the published sequences on Genbank.
They are generating a Microglossum phylogenetic tree with the publish species and their as yet unknown collections to see where they fit.
Mal