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14-05-2024 09:19

Hans-Otto Baral Hans-Otto Baral

Hi, I want to announce for next Sunday 17.00 middl

16-05-2024 11:19

Sylvie Le Goff

Bonjour, j'ai récolté sur une branchette de feui

16-05-2024 11:49

Castillo Joseba Castillo Joseba

De ayer en rama de abetoNo se por donde mirarA ver

14-05-2024 09:50

Yannick Mourgues Yannick Mourgues

Hi. Found on decorticated wod of Populus cf. alba

16-05-2024 09:02

Vasileios Kaounas Vasileios Kaounas

Found 15-05-24 in forest with Abies cephalonica, i

29-01-2022 21:44

Jan Eckstein

Good evening, apothecia small, yellowish, 150-300

15-05-2024 17:26

Vasileios Kaounas Vasileios Kaounas

First time I find this season and in this habitat,

16-05-2024 07:34

Vasileios Kaounas Vasileios Kaounas

Found in forest with Abies cephalonica. Diameter 0

15-05-2024 10:54

Viktorie Halasu Viktorie Halasu

Hello, would anyone have this paper please? I did

14-05-2024 19:48

B Shelbourne B Shelbourne

• Hymenoscyphus: Habitat, macro, spores, paraphy

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Niesslia-like Fruit Bodies but Microscopy Varies
Peter Thompson, 27-07-2017 15:30
Hello Everyone,

I have found fruit bodies which resemble the genus Niesslia macroscopically, but with differing microscopy.

The tiny, spherical fruit bodies have short, thick walled, pointed brown hairs and rest on the surface of their host.

Microscopically, the asci are arranged in whorls, containing eight pale brownish green spores, typically with one large guttule in each. The spores are in a single row in the asci and measure 8.5 - 10 x 5 - 6 um. The asci do not react to Melzers, but the spores inside them 'lose' their guttules.

Although the host is well decayed wood of Salix, there is an effuse greyish white layer covering its surface - possibly a resupinate from the basidiomycota. The conidial state of Chaetosphaerella phaeostroma is also present, thinly distributed, but right across the sample.

I have attached photos of the fruit bodies, asci and spores, hairs and effuse greyish matter.

I wonder if anyone has any ideas about what this tiny, hairy, black, spherical ascomycete might be?

Thank You,
Peter.




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Pascal RIBOLLET, 28-07-2017 14:30
Re : Niesslia-like Fruit Bodies but Microscopy Varies
Hello Peter,
Did you consider Helminthosphaeria ? The key by Miller et al. (2014) gives H. hyphodermiae, associated with resupinate basidio and with 1-celled spores...

Cheers
Pascal
Peter Thompson, 28-07-2017 16:01
Re : Niesslia-like Fruit Bodies but Microscopy Varies
Hello Pascal,

Thank you for pointing me towards Helminthosphaeria.

I will need to re-examine my sample to check for septa in some of the spores, because Miller et al, (2014) has led me to another document describing H. hyphodermiae and H. odontiae.

It seems very likely that the conidia and conidiophores, which I thought were those of the Diplosporium state of Chaetosphaerella phaeostroma, might instead be those of the Diplococcium state of Helminthosphaeria odontiae. The conidia and conidiophores of H. hyphodermiae seem different.

With Best Wishes,
Peter.

Peter Thompson, 31-07-2017 09:52
Re : Niesslia-like Fruit Bodies but Microscopy Varies
Hello Pascal,

I have looked at the sample again and see that some spores have a septum towards the base. This, the hairs with more pointed tips and the spore sizes clearly indicate that my sample keys out as Helminthosphaeria odontiae.

There seem to be no previous British records in either of the national databases.

With Best Wishes,
Peter.