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03-12-2021 16:37

maurice pelissier maurice pelissier

Bonjour trouvé dans les Hautes Pyrénées sur un

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Chris Yeates Chris Yeates

Bonjour tous An Ascobolus sp. has appeared in som

23-09-2021 13:40

Björn Nordén

Hi all,Does anyone by any chance have The taxonom

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Khomenko Igor Khomenko Igor

Hi, this fall around Ottawa, ON, Canada I found a

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Viktorie Halasu Viktorie Halasu

Hello,I have here a large Mollisia (approx. 3 mm,

02-12-2021 12:45

Stephen Mifsud Stephen Mifsud

I was revising some past identifications and I wis

29-11-2021 17:39

François Bartholomeeusen

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Joop van der Lee Joop van der Lee

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Bometon Javier Bometon Javier

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Chris Yeates Chris Yeates

Bonsoir tous As part of my study of the fungi occ

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Cosmospora species , Central Mediterranean region
Stephen Mifsud, 29-05-2017 12:45
Stephen MifsudI am reposting the finding of a Cosmospora species with new and more correct information.

This was growing on the wood pf chopped trunks of an unknown tree, just below the outer skin of the bark (hence not fully exposed). According to experts in this forum, the perithecia are embedded on the substratum of another Deuteromycetes fungus carpeting the log. I managed to grow pure colonies of the anamorph which I can't really tell if they are Fusarium or Fusarium-like Acremonium, but they are white cottony colonies.


DATA on TELEOMORPH:
=================



Ascocarp
Ascocarp morphology: Clustered ostiolate perithecia
Acocarp colour: Bright red, then blackening when old; purple-blue in KOH, orange-yellow in lactic acid
Ascocarp diameter: 0.16 – 0.32 mm (mean: 0.23 mm)
Ascocarp texture: Rough, Granulated or minutely tuberculate, ostiole with very fine setae
Ascocarp shape: Spherical then pyriform, opening at the apex by a tiny narrow ostiole sometimes widening to a cupulate shape


Paraphyses not numerous, filamentous, very thin and hyaline, inconspicuous and do not take the stain very well.


Ascum


Ascospore release:  through an apical orifice without an operculum
Asci: cylindrical to subclavate, slender, slightly bent ot curved, tapering gently towards the base.
No. of Spores 8
Tunic (Wall) Uni-tunicate, hyaline and do not stain well
Ascum length (range) 70 - 100 µm
Ascum length (mean) 84 µm
Ascum width (range) 6.8 - 7.5 µm
Ascum width (mean) 7.1 µm
Ascum L:W ratio 11.8
Iodine reaction (J +/J -) J-ve (but I'm not sure there is a blackish shadoe at the tip with IKI)
Orifice or ascum rim Rim slightly revolute, oriffice narrow, canal-like, surrounded by a thich asus wall.


Ascospores:
Spore length: (range) 11.46 - 16 µm
Spore length: (mean) 13.7 µm
Spore width: (range) 4.76 - 7.44 µm
Spore width: (mean) 6.3 µm
Spore Q factor (range): 1.84 - 2.85 µm
Spore Q factor (mean): 2.2
Spore shape: Fusiform to navicular with a constriction at the central septum
Spore septa 1

Spore surface: Smooth or finely rugose when observed at high resolution with LACB

Oil bodies: Two, one at each side of the septum, often seen as a small amorphous body of many aggregates coalescing together until finally forming a distinct globular oil body.
Remarks Spore quite variable in size.

Location: Malta, central mediterranean.

Your suggestions are welcome!

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Stephen Mifsud, 29-05-2017 12:50
Stephen Mifsud
Re : Cosmospora species , Central Mediterranean region
Is the the Anamorph an Acremonium or Acremonium-like Fusarium?

Images of colonies and microscopic data supplied:
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Stephen Mifsud, 29-05-2017 12:53
Stephen Mifsud
Re : Cosmospora species , Central Mediterranean region
I followed the keys of Rossman & Samuels: Genera of Bionectriaceae, Hypocreaceae and
Nectriaceae (Hypocreales, Ascomycetes) as follows:

1: On scale insects and adelgids : NO --> couplet 4
4: Ascomma superficial. orange. red to dark red, KOH+: anamorph not Fusorium merismoides : YES --> couplet 5
5: Ascospores > 10um long: YES (13.7um) -->22
22: Ascospores < 15um long: YES (13.7um) -->23
23: Ascospores > 4um wide: YES (6.3um) --> 32
32: Ascospores < 6um wide: borderline if NO -->33 / if YES -->43


33: Herbicolous. fructicolous. corticolous. or lignicolous. not fungicolous : NO, it is likely a fungicolous colony (although I still have a small doubt!) --> 36


36: Ascospores smoth to spinulose, verruuculous or tuberculate, not striate; anamorph
Fusarium or unknown; tropical and temperate regions: YES --> 38


38a:Ascospores tuberculate. 9.5-11(-12) x 5.5- 1(- 7.5) um, anamorph Acremonium-like.
with salmon colonies: NO, Ascospores longer
38b: Ascospores smooth to spinulose or verruculose: anamorph Acremonium-like Fusarium
or unknown: YES --> 39


39a. Perithecia with hyphal hairs arising from the surface of lhe perithecial wall, ascospores
12-13 x 5-7 um; anamorph unknown --> C. rubrisetosa : I don't think there are hyphal hairs and ascospores longer, not so sure!!!
39. Perithecia glabrous: ascospores 9.5-15 X 4.5- 7 um; anamorph Acremonium-like or
Fusarium: Probably YES --> 40


40a: Ascosporcs (10-)11.5-14.5(-16) x (5-)5.5-7.5(-10) um. smooth to slighlly spinulose; anamorph Acremonium-like. On wood possibly fungicolous, central and northern Africa --> C. meliopsicola.


40b. Ascospores 9.5-15 x 4.5-7.0um spinulose; on immersed ascomyceles; anamorph
Fusarium from north temperate New Zealand --> 41

I'm excluding lead 40b because spores are not spinulose, distribution region does not match.


Any one famigliar with C. meliospicola ?

Stephen Mifsud, 29-05-2017 13:20
Stephen Mifsud
Re : Cosmospora species , Central Mediterranean region
32: Ascospores < 6um wide: borderline (6.3um) so if this is taken YES -->43


43a: Perithecia with a fringe of hyphal hairs around the apex; ascospores
(12- )13-16.5(- 17) x (5.5 - )6-7.5(- 8) um; anamorph Fusarium; On non-valsaceous
fungi C. pseudoflavoviridis: No hyphal hairs, not on fungi : NO
43b: Perithecia glabrous  or with setae surrounding the ostiole: ascospore, 8-18.5 X 4-8 um: OK --> 44


44a:  Petithecia with setae surrounding ostiole: Ascospore. 12-18 x 4-8 um: anamorph
Fusarium: grows on bark; New Zealand -->  C. dinglae.  Fungicolous and not new Zealand so NO
44b. Perithecia glabrous: anamorph Aremonium-like Fusarium, or unknown: on Xylariaceae or wood; temperate or tropical regions --> 45 YES

45a Europe or United Stales (New Jersey): YES --> 46 
45b Tropical America. central and southcrn Africa NO

46a. On Valsa sorbi, Europe: ascospores (13- )13.5- 16.5{-18.5) x (5.5- )6-7(- 7.5) )um;
anamorph Fusarium  -->  C. stilbosporae  
46b. On dead. scale-like leaves of Chamaecyparis possibly on immersed asoomycetes: United
Stales (New Jersey): ascospores 8-15 x 7-8 um, anamorph unknown --> C. thujana (NO)



CONCLUSION

C. meliospicola if width of spores is > 6um; African region
or
C. stilbosporae if width of  spores is < 6um; European but on Valsa sorbi ?? (What is this??)


Stephen Mifsud, 08-06-2020 10:19
Stephen Mifsud
Re : Cosmospora species , Central Mediterranean region
I am bumping this post wondering if someone is interested in this collection and have found something similar.