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Sarcoscypha coccinea?
Viktorie Halasu, 15-03-2017 18:39
Viktorie HalasuHello,
I'm trying to determine a Sarcoscypha:
Apothecium ca. 2,5 cm wide with crenulate margin.
Spores 26-32 × 9,6-12,4 um, long ellipsoid, usually with rounded ends, only sometimes truncate but not distinctly saddle-shaped. Two polar groups of small LBs. Immature spores in ascus have a sheath all aroud. Only a few spores germinating, always just from one end. 
Hairs very sparse near the margin, at the base of apotecium long and straight or wavy hairs. 

Host: I think it is Fraxinus excelsior or angustifolia (it's from southern Moravia, one of the warmest parts of Czech Rep., where both species live).  
With the key I arrived to coccinea, but still have some doubts, partly because of the host. 

Thank you for any advice.
Viktorie
  • message #47919
  • message #47919
  • message #47919
Nicolas VAN VOOREN, 15-03-2017 18:45
Nicolas VAN VOOREN
Re : Sarcoscypha coccinea?
For me it's clearly S. coccinea (spores and external hairs)
Viktorie Halasu, 15-03-2017 18:52
Viktorie Halasu
Re : Sarcoscypha coccinea?
Hello Nicolas,
thank you for confirmation.
Hans-Otto Baral, 15-03-2017 18:55
Hans-Otto Baral
Re : Sarcoscypha coccinea?
I also agree (although the entire spore sheath I remember only for jurana).

Host is Fraxinus, yes.

Would it be possible to know more precisely the collecting place?


Thanks
Zotto
Viktorie Halasu, 15-03-2017 19:45
Viktorie Halasu
Re : Sarcoscypha coccinea?
Hello Zotto, 
thank you, I'll send you the collection data.

Yes, the sheath is interesting. At first, I thought about S. jurana (disregarding the host). I've even found something like conidia with the right size (ca. 7,5-10,6 × 2,3-3,3 um). But I didn't see any big LBs in living spores and the most truncated spores are on this photo (all ejected in the preparate). And I also didn't see any connection between these conidia and spores. So now I think it might be rather a contamination from the collection site. 

About the conidia - could they develop after a few days in the fridge? And where to look for them - is any part of the hymenium better than the other (center vs. margin)?

Viktorie
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Hans-Otto Baral, 15-03-2017 20:35
Hans-Otto Baral
Re : Sarcoscypha coccinea?
any part of the hymenium is good and yes, they may develop in the fridge. But S. coccinea develops conidia only on an agar plate.

As a sheath  one may mistake the spore wall when the spores are dead. In your three pics below I am not sure, they look a bit strange, maybe they are submature. When mature then the central area looks empty (but contains many nuclei).