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21-05-2025 17:28

éric ROMERO éric ROMERO

Bonjour Tous, Avec la bonne fiche cette fois (mer

22-05-2025 08:55

Josep Torres Josep Torres

Hello.A Pezizal sprouting scattered in small group

22-05-2025 17:17

Per Marstad Per Marstad

I have found this on Alnus glutinosa. Growing unde

19-05-2025 09:42

Josep Torres Josep Torres

Hello.An ascomycete photographed on April 20, spro

21-05-2025 08:31

Josep Torres Josep Torres

Reddish-brown, grain-like ascomata, measuring 0.7â

20-05-2025 09:37

Josep Torres Josep Torres

Hello, an anamorph photographed last April 20th, s

20-05-2025 22:15

Francois Guay Francois Guay

I found this ascomycete at the base of a dead fern

20-05-2025 21:36

Francois Guay Francois Guay

I found this Mollisia sp. on a submerged herbaceou

20-05-2025 10:31

Castillo Joseba Castillo Joseba

me mandan elmaterial seco de Galicia, (España) 

16-05-2025 05:47

Francois Guay Francois Guay

I found this super tiny hyaline asco on fir needle

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is this Orbilia rubrovacuolata?
François Bartholomeeusen, 16-02-2016 21:31
Hi all,

Found on dead stalk of Rubus lying on the ground:

FRB: ruby red, < 1.7 mm, dry: flat and semitranslucent, moist: swollen, cushonlike 
Asci :36,42-37,92 x 4,03-4,61 µm,
Sspores: filiform strongly curved 7,80-11,89 x 0,82-1,69 µm (length with curve: 11,94-17,52 µm)
Paraphyses: filiform, apices swollen, knoblike
Texture: globulosa 

Thanks to this fantastic site I can determine these apothecia: orbilia rubrovacuolata. Is that correct?
Orbilia's frustrate me, they confront me with my own shortcomings on mushroom knowledge and with the shortcomings of my microscope. With oil-immersion and a magnification of 1000 I am unable to get sharp photos.
I have no literature on this asco, so am I right?

Greetings,

François Bartholomeeusen
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Michel Hairaud, 16-02-2016 21:57
Michel Hairaud
Re : is this Orbilia rubrovacuolata?

Bonsoir François,


Oui, Ascofrance est un outil formidable pour chacun d'entre nous et nous avons tous aussi la chance que Zotto ait généreusement diffusé ses connaissances par ses DVD et son site. 


Juste une rapide remarque avant que Zotto n'apporte des précisions (notamment sur la documentation) car à ma connaissance cette espèce n'est pas encore publiée mais connue de beaucoup de mycologues. Pour ma part , je l'ai récoltée en Normandie, en Bretagne et en Asturies , soit le long de la côte atlantique , mais pas encore dans ma région du Centre Ouest. Cette récolte est elle aussi proche de la côte ?


La couleur des paraphyses et la forme des spores concordent bien, mais la couleur des apothécies me semble un peu dénaturée, peut être par un renfort du contraste ?


Amitiés. Michel

Hans-Otto Baral, 16-02-2016 22:12
Hans-Otto Baral
Re : is this Orbilia rubrovacuolata?
Cher Francois

sans doubt!

Your microphotos are not bad, and I do likewise take my pics at 1000x oil, without using the zoom. Is your spore photo done with 400x? I could imagine that you need perhaps more light to achieve a shorter shutter speed which makes tremblng spores sharper.

It is true what Michel says, the species was not yet found in Charente-Maritime, but it starts to occur just a bit north (see map).

I would be pleased to include your collection data in the monograph. Perhaps you have coordinates of the place?

Zotto
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François Bartholomeeusen, 16-02-2016 22:40
Re : is this Orbilia rubrovacuolata?
Bonsoir Michel,

Grâce à la couleur frappante de l'apotheses je suis réussi à trouvée les ascos sur terre sur une vieille tige de rubus, près de chez moi.
HA la belle France.... mais j'habite en Belgique, Anvers (province) dans la ville de Turnhout, à environ 200 km de la côte belge. Ci joint deux photos de l'apothesia.

Merci pour votre reponse,

François
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François Bartholomeeusen, 16-02-2016 22:48
Re : is this Orbilia rubrovacuolata?
Hi Zotto,

Indeed the picture with spores is taken with magnification 400 x. Attached a picture with magnification 1000 x. It seemed as if the spores were swimming like an amoeba, so difficult to photograph! In annex the coordinates:

Found on 15 februari 2016, Belgium, province of Antwerp, city Turnhout in the nature reserve Frans Segers in Turnhout
WGS84 lat 51.297026201583286 – lng 4.923405871450086
IFBL B55723

Thanks en good evening,

François
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François Bartholomeeusen, 16-02-2016 22:51
Re : is this Orbilia rubrovacuolata?
Foregotten...the picture
Hans-Otto Baral, 16-02-2016 22:56
Hans-Otto Baral
Re : is this Orbilia rubrovacuolata?
This is good indeed, thanks!

my shutter speed is about 1/30 or even shorter, and herewith it does not trouble when spores move. But the problem I see on your photo is rather that the spores do not lie in a plane. This is almost unavoidable. Usually the spores settle on the bottom after some time and then it will produce better photos.

By the way, your hyperexact values are perhaps too wide, I never saw spores outside the range of (8.5–)9–12(–13) × (0.8–)0.9–1.2(–1.3).

Michel Hairaud, 16-02-2016 23:39
Michel Hairaud
Re : is this Orbilia rubrovacuolata?

Merci François,


Hi Zotto,


I guess from your map that the most French southern collection in in Vendée . Is that correct ? Otherwise, it seems that the species would rather prefer ''colder '' areas (acid soil ?), which may explain the absence from the South West coast. (unless it shows the absence of mycologists interested in Orbilias)


Michel

Joey JTan, 16-02-2016 23:52
Re : is this Orbilia rubrovacuolata?
Getting high quality images of ascospores floating in water can be frustrating at times. 

I sometimes allow ascospores to shoot onto (or make mounts in) a drop of 1% methyl cellulose solution placed on a slide.  It is viscous, effectively slowing down the ascospore movement and making photography easier, and does not seem to affect spore dimensions or the vitality of the ascospores.
François Bartholomeeusen, 17-02-2016 07:31
Re : is this Orbilia rubrovacuolata?
Hi Joey,

Thanks for the tip; I will have to expand my chemical collection!

Greetings,

François
Hans-Otto Baral, 17-02-2016 09:02
Hans-Otto Baral
Re : is this Orbilia rubrovacuolata?
Hi Michel

I have a simplified geological small map of entire Europe showing cretaceous and tertiary sediments in Aquitaine and Cambrian in Brittany. It may well be that acidity of the soil plays a role. But some collections appear to fall in Cretaceous areas.

It is true that the southermost samples in France are from Vendee. maybe the fungus does not like the Charente-Maritime area. :-)

From the climate I think Vendee and Charente-Maritime are too similar to explain the absence.

Francois: if you keep the sample in a herbarium please tell me the number.

Zotto
François Bartholomeeusen, 19-02-2016 16:01
Re : is this Orbilia rubrovacuolata?
Guten Tag Zotto,

Fast vergessen! Die Seriennummer des Exsicaat in meinem Herbarium: 20160215FS01

Mit freundlichen Grüßen,

François
Hans-Otto Baral, 19-02-2016 16:23
Hans-Otto Baral
Re : is this Orbilia rubrovacuolata?
Vielen Dank!

In der Einleitung zur Monografie erklären wir die verwendeten Herbarabkürzungen und geben da immer den Wohnort des Finders an. Is es so richtig?


F.B. = F. Bartholomeeusen (Turnhout) ?


Gruß
Zotto
François Bartholomeeusen, 19-02-2016 18:21
Re : is this Orbilia rubrovacuolata?
Ganz korrekt,

Grüßen,

François