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Mark Stevens

"Hello everyone,I'm relatively new to microscopy (

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Hello everyone, Is it possible to at least approx

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Hymenoscyphus? on Picea abies
Marja Pennanen, 19-10-2015 00:39
Hello forum.

these Hymenoscyphuslike grow on Picea abies and there mostly on polypore Trichaptum abietinum.
They are about 1-3 mm wide.
The spores are multiguttulate, 14-20x5-7+.
The asci are about 130-150x10-12 and paraphyses about 3-4 wide at the top.

There have been frosty nights here and some ascomycetes survived throught them.
Once again I'm lost trying to determine these, but if some more information is needed, I can make more preparates.

As you can see, good habitats are wanted and in my opinion Chaenotheca brunneola has found that too ;)

Marja
  • message #38450
  • message #38450
  • message #38450
Hans-Otto Baral, 19-10-2015 10:38
Hans-Otto Baral
Re : Hymenoscyphus? on Picea abies
Hi Marja

did you check the iodine reaction? Paraphyses guttulate?
Marja Pennanen, 19-10-2015 17:16
Re : Hymenoscyphus? on Picea abies
Hi Zotto,

asci are weakly IKI+.
Some paraphyses had VB:s, The paraphyses of a yonger fungus, that I studied were narrower at the tips and asci can go over 150. I wonder weather this is a Hymenoscyphus at all.

These really grow only on the area of the polypore and mostly on the polypores or its remnants.

Marja
  • message #38461
  • message #38461
Hans-Otto Baral, 19-10-2015 17:24
Hans-Otto Baral
Re : Hymenoscyphus? on Picea abies
Ahhhhhhh. The apical ring is of the Calycina-type. I remember Calycina (Bisporella) citrina growing on a polypore, it was the type of Parthenope parasitica that turned out to be C. citrina on Gloephyllum abietinum.

Such large spores would go to Bisporella confluens, though 5-7 µm would be too wide.
Marja Pennanen, 19-10-2015 20:09
Re : Hymenoscyphus? on Picea abies
I wondered the wideness of spores, too.
They really start from 5 and the wider spores are really even over 7.
Here's an example.

Marja
  • message #38468
Hans-Otto Baral, 19-10-2015 22:38
Hans-Otto Baral
Re : Hymenoscyphus? on Picea abies
Did you study the excipulum?
Marja Pennanen, 20-10-2015 18:16
Re : Hymenoscyphus? on Picea abies
Yes, but the photos were horrible. So I made another preparate.
  • message #38480
  • message #38480
Hans-Otto Baral, 20-10-2015 18:26
Hans-Otto Baral
Re : Hymenoscyphus? on Picea abies
Looks like a thin-walled t. porrecta, but I am not sure if the cell walls would become thicker when killed. You didn't use oil immersion?
Marja Pennanen, 20-10-2015 19:42
Re : Hymenoscyphus? on Picea abies
Hi.
I use oil immersion with 1000x, just that the microscope is an old one, that has been used to retirement in Turku.
Maybe I will make one more preparate and make some detail photoa, if the mission is not impossible ;)

Marja
Hans-Otto Baral, 20-10-2015 19:48
Hans-Otto Baral
Re : Hymenoscyphus? on Picea abies
But those excip. photos are made with 400x.
Marja Pennanen, 20-10-2015 20:23
Re : Hymenoscyphus? on Picea abies
Ooh yes, that is possible.
The new operating system has decided to put photos to month folders (previous used day folders and that was fine) and now my photos are a mess with different photo dublicates or triplicates or ... on same names and so on ... I got to rename the folder every time after importing photos for now on to keep them on some kind of order.
Everything is a bit different and difficult after updates to data systems...

I'll make a new preparate and try to keep the photos in their own folder, so I can find what I'm looking. Be back later.

Marja
  • message #38488