27-02-2026 11:21
Yannick Mourgues
Hi to all. Here is a specie that can may be relat
26-02-2026 15:00
Castillo Joseba
Me mandan el material seco de Galicia, recolectada
24-02-2026 11:01
Gernot FriebesHi,found on a branch of Tilia, with conidia measur
23-02-2026 11:22
Thomas Læssøehttps://svampe.databasen.org/observations/10584971
29-11-2024 21:47
Yanick BOULANGERBonjourJ'avais un deuxième échantillon moins mat
07-02-2023 22:28
Ethan CrensonHello friends, On Sunday, in the southern part of
19-02-2026 17:49
Salvador Emilio JoseHola buenas tardes!! Necesito ayuda para la ident
Demande
René Dougoud,
27-05-2015 09:48
J'ai trouvé dans Mycologia 71, 1979, un article de R.J. Bandoni: Safranin O as a rapid nuclear stain for fungi. (chez Cyberliber)
Ma demande : Avez-vous de l'expérience avec ce colorant nucléaire ?
Merci de vos réponses
René
Carmel Sammut,
28-05-2015 18:41
Re : Demande
Hi René,
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
