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Garcia SusanaEste otro crecía en el mismo trocito de madera qu
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Edvin Johannesen
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Filip Fuljer
Hello everyone,a few days ago I collected this str
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William Slosse
Good evening everyone, On 18/03/26 I found a few
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François Freléchoux
Bonjour, Voici la description rapide d'un petit d
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Enrique Rubio
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Khomenko Igor
I recently examined Celtis occidentalis branches
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Bernard CLESSE
Bonsoir à toutes et tous,Pourriez-vous m'aider à
Demande
René Dougoud,
27-05-2015 09:48
J'ai trouvé dans Mycologia 71, 1979, un article de R.J. Bandoni: Safranin O as a rapid nuclear stain for fungi. (chez Cyberliber)
Ma demande : Avez-vous de l'expérience avec ce colorant nucléaire ?
Merci de vos réponses
René
Carmel Sammut,
28-05-2015 18:41
Re : Demande
Hi René,
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
