14-04-2026 21:52
Gernot FriebesHi,found on dead leaves of Carex elata. Conidia: 4
14-04-2026 20:31
Gernot FriebesHi,can this be Psilachnum lateritioalbum on Phragm
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Ethan CrensonHi all, A few weeks back a friend pointed out som
12-04-2026 17:56
Hardware Tony
Found on dead stems in February earlier this year
12-04-2026 15:52
Gernot FriebesHi,I'm looking for help with this anamorph collect
12-04-2026 12:22
William Slosse
In a dune grassland in Oostduinkerke (Belgium), on
11-04-2026 15:45
Zuzana Sochorová (Egertová)
Please, could anyone send me this paper?Moyne G.,
11-04-2026 13:34
Artem PtukhaHello, I am seeking assistance with the identific
11-04-2026 10:19
Michel Hairaud
Chers amis d'Ascofrance , voici une très bonne no
Demande
René Dougoud,
27-05-2015 09:48
J'ai trouvé dans Mycologia 71, 1979, un article de R.J. Bandoni: Safranin O as a rapid nuclear stain for fungi. (chez Cyberliber)
Ma demande : Avez-vous de l'expérience avec ce colorant nucléaire ?
Merci de vos réponses
René
Carmel Sammut,
28-05-2015 18:41
Re : Demande
Hi René,
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.



