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Alex Akulov
Dear FriendsToday I made the pdf file of Velenovsk
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Lothar Krieglsteiner
... on twig in the air at standing Ceratonia siliq
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Stephen Martin Mifsud
ID request for what seems to be a true aquatic fun
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Stephen Martin Mifsud
ID request: This specimen was collected in Malta o
04-05-2026 09:50
Castillo Joseba
Me mandan el material seco de Galicia,(España) re
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Alain BRISSARDBonjour à tousJeuidi 30 avril dernier on m'a remi
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Pauline. PennaBonjour Please can someone help me with this id
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Thierry Blondelle
Bonjour à tous, Une récolte sur bouse séchée d
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Ethan CrensonHi all, A few weeks back a friend pointed out som
Demande
René Dougoud,
27-05-2015 09:48
J'ai trouvé dans Mycologia 71, 1979, un article de R.J. Bandoni: Safranin O as a rapid nuclear stain for fungi. (chez Cyberliber)
Ma demande : Avez-vous de l'expérience avec ce colorant nucléaire ?
Merci de vos réponses
René
Carmel Sammut,
28-05-2015 18:41
Re : Demande
Hi René,
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
