20-12-2025 23:08
Patrice TANCHAUDBonsoir, récolte sur sol sablonneux dans l'arriÃ
21-12-2025 09:32
Josep Torres
Hello.A tiny ascomycete found embedded in wood in
20-12-2025 15:47
Mirek GrycHi.These grew on pine wood that was heavily covere
18-12-2025 21:17
Pol DebaenstThe identification took me to Byssonectria deformi
15-12-2025 07:09
Danny Newman
indet. Rutstroemiaceae sp. on unk. fallen leavesMc
19-12-2025 10:10
Patrice TANCHAUDBonjour, récolte réalisée en milieu dunaire, a
18-12-2025 17:23
Bruno Coué
Bonjour,je serais heureux d'avoir votre avis sur c
Demande
René Dougoud,
27-05-2015 09:48
J'ai trouvé dans Mycologia 71, 1979, un article de R.J. Bandoni: Safranin O as a rapid nuclear stain for fungi. (chez Cyberliber)
Ma demande : Avez-vous de l'expérience avec ce colorant nucléaire ?
Merci de vos réponses
René
Carmel Sammut,
28-05-2015 18:41
Re : Demande
Hi René,
         I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
         I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.
I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
