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14-04-2026 21:52

Gernot Friebes

Hi,found on dead leaves of Carex elata. Conidia: 4

14-04-2026 20:31

Gernot Friebes

Hi,can this be Psilachnum lateritioalbum on Phragm

14-04-2026 05:32

Ethan Crenson

Hi all, A few weeks back a friend pointed out som

12-04-2026 17:56

Hardware Tony Hardware Tony

Found on dead stems in February earlier this year

12-04-2026 15:52

Gernot Friebes

Hi,I'm looking for help with this anamorph collect

12-04-2026 12:22

William Slosse William Slosse

In a dune grassland in Oostduinkerke (Belgium), on

11-04-2026 15:45

Zuzana Sochorová (Egertová) Zuzana Sochorová (Egertová)

Please, could anyone send me this paper?Moyne G.,

11-04-2026 13:34

Artem Ptukha

Hello, I am seeking assistance with the identific

11-04-2026 10:42

Castillo Joseba Castillo Joseba

Me mandan el material de Galicia, España, recolec

11-04-2026 10:19

Michel Hairaud Michel Hairaud

Chers amis d'Ascofrance , voici une très bonne no

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Demande
René Dougoud, 27-05-2015 09:48
Chers Tous,

J'ai trouvé dans Mycologia 71, 1979, un article de R.J. Bandoni: Safranin O as a rapid nuclear stain for fungi. (chez Cyberliber)

Ma demande : Avez-vous de l'expérience avec ce colorant nucléaire ?

Merci de vos réponses

René
Carmel Sammut, 28-05-2015 18:41
Re : Demande
Hi René,
          I don't know if this is of any help. I have tried using o-safranin as a counterstain with toluidine blue. It didn't work but I use this mix for thin structure like paraphyses (in the attached photo from Propolis farinosa). It tends to overstain quickly.

I tried simple staining with o-safranin alone but I am not sure what I should see. I rarely use acetocarmine stains as I get mixed results with precipitates and unclean slides. I am attaching some tests on a Tarzetta - cupularis or gaillardiana (some spores are in the 26-27µm range). The stain was a 1% water solution added near the coverslip.
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