07-06-2015 11:59
Maren Kamke
Hi everybody,Apothecia messures up to 0,5 mm in di
07-06-2015 23:15
Steve ClementsHi,This stromatic pyrenomycete was found on a beec
07-06-2015 22:16
Esquivel-Rios EduardoSaludosEncontre este hongo en un suelo organico, p
07-06-2015 19:37
Chris Yeates
Bonsoir tousthis fungus was collected in good cond
28-05-2015 19:45
John LeachHi all I recently found this Ascomycete in Anston
05-06-2015 21:36
Peter ThompsonHello Everyone,I have found some grey perithecia i
06-06-2015 15:00
Bernard CLESSE
Bonjour à tous,Voici ce que je pense être un Das
06-06-2015 20:04
Chris Yeates
Bonsoir tousFollowing my coming across "Psilachnum
06-06-2015 20:25
Quijada LuisHi all,I would like to know if somebody in the for
Thank you in advance,
zaca
Data:
A few apothecia growing on soil under shrubs;
Spores:
(13.8) 16.1 - 18.8 (19.7) x (10) 11.5 - 13.8 (15.1) µm
Q = (1.3) 1.33 - 1.49 (1.5) ; N = 50
Me = 17.4 x 12.4 µm ; Qe = 1.4
Asci: 220-260 x 12-16 µm;
Paraphysis tips globose up to 10 µm in diameter;
Hairs up to 350 x 32 µm.
How are you ?
For this collection, impossible to say without spores in BC...
Probably around nigrohirtula complex, but spores seem to be not mature...
Beñat
I hope everything is right with you as well.
I suppose that by BC you mean "Bleu de Crésyl". I posted some photos of it in the 4th group; The stain was not very effective; Maybe I have to let the blue act for a litle longer. Anyway, I will do it again (in the last apothecia I have, the collection was really short) and post here the results.
Best regards,
zaca
The usual stain for Scutellinia spores is Lactophenol Cotton Blue.
Mal
But, I experienced the same problem with Lactophenol Blue than before with Crésyl Blue, the stain is poor and is unevenly distributed. I upload the result of this try.
Regards,
zaca
but as Beñat has pointed out the spores are immature and therefore cannot be of any help. See if you can make a spore drop - with a (thin) marker pen draw a small circle on a microscope slide; turn the slide over and place the hymenial surface of the Scutellinia directly over the circle.
If you leave that for a good period of time you can check the dry microscope slide for ejected (mature) spores in the area within the circle under the compound microscope at x100 or x200; if you see spores, mount in Cotton Blue in Lactophenol, heat gently and show the results.
I hope Beñat does not disagree too much with this procedure I often use . . . .
amitiés
Chris
for your detailed explanation that certainly will be helpful in future.
Unfortunately this time it didn´t work: in fact, after an entire day I was not able to see any spore. The material was already very scarse.
Regards,
zaca