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Cheilymenia denisii 070614
Miguel Ángel Ribes, 06-08-2014 09:20
Miguel Ãngel RibesGood morning

I know August is a holiday month, but now I have time to send this species.

This Cheilymenia was in a snowfield (see first picture) and I think there is not doubt about his identification, but I prefer your opinion.

Spores eliptical and slightly ornamented, size (18.6) 19.3 - 21.9 (22.5) x (10.3) 10.6 - 11.7 (12.9) µm; Q = (1.6) 1.7 - 2.0 (2.2) ; N = 91; Me = 20.6 x 11.2 µm ; Qe = 1.9.

Hairs size (222.7) 235.0 - 401.2 (435.2) x (16.4) 19.1 - 27.5 (37.2) µm
N = 27; Me = 319.6 x 23.9 µm

Moniliform paraphysis and croziers.

Hifal system at base very developed. 

Thank you for your opinion
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Michel Delpont, 06-08-2014 09:52
Michel Delpont
Re : Cheilymenia denisii 070614
Holla Miguel.

You do not specify the substrate, but it looks good on the picture that there is dung. This is a difficult species to determine, but I think C.denissi corresponds to your description. Have you also checked the length of asci?

Michel.
Miguel Ángel Ribes, 06-08-2014 11:24
Miguel Ãngel Ribes
Re : Cheilymenia denisii 070614
Upsss, I am sorry Michel

Of course, substrate is cow dung, and this are measures of living asci at 600x:
(267.5) 280.0 - 310.6 (326.3) x (17.1) 17.6 - 20.4 (21.2) µm; N = 24; Me = 295.6 x 19.1 µm


Why is so difficult to determine this species? According to Moravec, the nearest species is C. liskae, who have shorter hairs (90-300 x 10-30), bi, tri o multifurcate; asci shorter and wider (150-180x18-27) and longer spores (18-22,5 x 10-13).


The doubt is that Moravec spores measure for C. denisii are 14-21 x 8-13,5, and asci 170-240 x 13,5-18, both shorter than mine, perhaps died material?


Thank you Michel

Hans-Otto Baral, 06-08-2014 12:25
Hans-Otto Baral
Re : Cheilymenia denisii 070614
Hi Miguel

surely the asci show such enormous shrinkage as is seen between your and Moravec's ascus sizes. The spore data are more equal when considering perhaps immature spores included in Moravec's data. Moravec probably mostly measured dead elements as far as I know his papers.

I have only a few pages of Moravec's monograph. On Fig. 23 I wonder why he figured a dead (b) and a living ascus (c) and addressed them to different varieties. Was he unaware of ascus shrinkage?

In your sample I am impressed especially of the carotenoid crystals in the paraphyses. I know this feature only in Scutellinia, and in one species of Cheilymenia (crucipila), which was later transferred to Scutellinia, I think for molecular reasons.

Maybe Cheilymenia dennisii is also a masked Scutellinia despite of the low lipid content in the spores?
 
Zotto
Miguel Ángel Ribes, 06-08-2014 12:57
Miguel Ãngel Ribes
Re : Cheilymenia denisii 070614
Yes, I saw irregular edge in spore, paraphysis and asci drawing at Moravec monograph, so I assumed they were dead, but I was not sure.

I think my measures are on living state (attached picture).

Another discrepance with Moravec is about croziers, he said no croziers, and I think mine has it.

Very interesting hypothesis about Cheilymenia / Scutellinia ¿?, of course I have no answer.

Thank you
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Hans-Otto Baral, 06-08-2014 12:59
Hans-Otto Baral
Re : Cheilymenia denisii 070614
Yes, these are alive. 

But I was wondering about the ascus base, it looks to me like lacking croziers.
Miguel Ángel Ribes, 06-08-2014 13:33
Miguel Ãngel Ribes
Re : Cheilymenia denisii 070614
Ok so, you have more experience.

Thanks.