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07-11-2018 08:34

Zuzana Sochorová (Egertová) Zuzana Sochorová (Egertová)

Hello, could someone send this publication to me

18-05-2024 10:47

Anna Klos

Goodmorning,We found this tiny ascomycete (max. 1m

25-03-2024 19:14

Juuso Äikäs

I found some small black pyrenos growing on a dead

14-04-2024 20:04

Manak Roman

Hi all,I have two very similar finding last weeken

10-05-2024 17:40

Anna Klos

Good afternoon, Thursday during an inventory we f

17-05-2024 15:17

éric ROMERO éric ROMERO

Bonjour, Un besoin d'aide pour ce Podospora (...?

17-05-2024 16:25

Pavel Jiracek

Erioscyphella lunata, found on a fallen needle of

29-01-2022 21:44

Jan Eckstein

Good evening, apothecia small, yellowish, 150-300

16-05-2024 11:19

Sylvie Le Goff

Bonjour, j'ai récolté sur une branchette de feui

14-05-2024 09:19

Hans-Otto Baral Hans-Otto Baral

Hi, I want to announce for next Sunday 17.00 middl

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Unknown Stictidaceae sp.
Joey JTan, 24-07-2014 21:23
I collected this specimen from a dead lower branch on a mature red spruce (Picea rubens).  I assumed it was a Stictis sp. but a quick look under the microscope proved otherwise. 

The apothecia are about 1 mm in diameter and immersed in the host tissue.  They erupt from the bark and have a stellate appearance; the hymenium looks granular/powdery with a hand lens.

The subhymenium is colourless and appears to be emerging directly from the host tissue.  The hymenium is full of crystals and paraphyses are filiform, often branched, and look knobby/swollen at the apices.  Asci are cylindrical, somewhat thick-walled, J- (Melzer's), and I could not see a defined apical apparatus.  The ascospores are broadly epllipsoidal, avg. 25 x 10 um. 

I apologize for the image quality, I have lots of specimens to go through and very little time! 

Thank you very much,

- Joey
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Chris Yeates, 24-07-2014 21:39
Chris Yeates
Re : Unknown Stictidaceae sp.
Hi
surely not Stictidaceae, the spores and particularly the ascus apex are inconsistent with that family. Image quality is pretty good ;)
regards
Chris
Joey JTan, 24-07-2014 21:45
Re : Unknown Stictidaceae sp.
Yes, not a Stictidaceae sp.  I was thinking it may have been treated as such in the old literature (e.g.: Stictophacidium sensu Sherwood).

Thanks
Hans-Otto Baral, 24-07-2014 21:47
Hans-Otto Baral
Re : Unknown Stictidaceae sp.
Were the spores with two large guttules when alive? Is the spore photo on water?
My guess is a Propolis. Asco should be negative also with Lugol (Melzer is not good, gives also often negative reaction in Stictidaceae).

But I wonder about the crystals, maybe very small ones among the paraphysis tips?

Joey JTan, 24-07-2014 21:53
Re : Unknown Stictidaceae sp.
The spore photos were done in water.  I do not have an image of it, but I did see some immature spores in an ascus with two large polar guttules.

Propolis is probably the best place to start, thank you.
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Joey JTan, 24-07-2014 22:08
Re : Unknown Stictidaceae sp.
Hans,

I made another cross section mounted in water and noticed many (living) spores with larger aggregated guttules than those in my first picture, however I did not see any with two large guttules.

The crystals are quite interesting.  They appear to be rather uniformly distributed throughout the hymenium and I have not seen them associated with the tips of the paraphyses.
Hans-Otto Baral, 24-07-2014 22:13
Hans-Otto Baral
Re : Unknown Stictidaceae sp.
In your fifth picture I think I see this mass of crystals. But is it that they disappear in melzer? Or why are they not shown on the closeups? Are they really crystals, because there exists also oleaginous substances that are interspersed in the hymenium in some taxa.

If the spores are multiguttulate  then this is a good charcater because in Propolis only a few have it. But I know only such with broadly ellipsoid spores, e.g. P. leonis.
Joey JTan, 24-07-2014 22:39
Re : Unknown Stictidaceae sp.
I am not entirely sure if they are crystals or a refractive oleaginous substance as you mention.  When Melzer's was added to a mount made in water, the "crystals" disappeared.

P. leonis has been found on Picea, so it may be a good place to start.  Thank you very much.
Hans-Otto Baral, 24-07-2014 23:00
Hans-Otto Baral
Re : Unknown Stictidaceae sp.
Oxalate Crystals resist in all current mountanst such as MLZ or KOH. 

Maybe all Propolis have such substance. In what I named P. rhodoleuca I also saw only small drops.

But what I take as P. versicolor may also occur with rather small drops (microphoto attached)
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