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Sylvie Le Goff

Bonjour Peut on dire que les poils soient vitreux?

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Hello,I failed to identify this anamorph, which gr

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Hi again Could you help me with this paper? NANN

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Sclerotinia tetraspora
Nina Filippova, 08-06-2014 13:00
I have seen sclerotia of, likely, this species several times before in stems of Rubus chamaemorus. Apothecia were found for the first time, in this case they arise from a berry of the same plant. Date of collection: 02.06.2014. Coordinates: N60,891704° E68,684973°


Apothecia cupulate with long stipe, up to 2.5 cm long, disc 2.5 mm across, light brown, hymenium light yellowish-brown, upper part of stipe pubsecent under lens, arise from short cylindric sclerotia, 5 apothecia collected from one old berry, sclerotia attached to seeds.


Asci cylindrical with narrowed base, with crozier, with euamyloid ring, 4-spored (but irregularly one to 6-spored asci seen, with part of spores abrupt, many of asci were also underdeveloped and empty), 108-119 x 9.5-11.3 mk; paraphyses cylindrical, moderately branched, with many small greenish guttules, 2.4-3.7 mk broad in upper part; spores ellipsoid, inequilateral, with two small guttules at both ends, 13.1-15.5 x 6-7.5 mk (n=7, only large not abrupt spores measured).

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Nicolas VAN VOOREN, 09-06-2014 08:47
Nicolas VAN VOOREN
Re : Sclerotinia tetraspora
Can you describe the structure of excipulum?
Nina Filippova, 09-06-2014 11:50
Re : Sclerotinia tetraspora
Of course,

outer layer of receptacle from textura globosa, cells with thickened walles, yellowish, end cells at the edge clavate.

Stipe has excipulum from textura porrecta, cells brown, slightly rough from incrusting pigment, with branching hyphoid hairs.
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Hans-Otto Baral, 09-06-2014 23:10
Hans-Otto Baral
Re : Sclerotinia tetraspora
In my database I find on R. chamaemorus

Rutstroemia chamaemori
Ciboria latipes 
Sclerotinia tetraspora

all with a similar spore size. 

Did you compare all three? I do not know either of them but I suspect you are right with Sclerotinia tetraspora, because latipes does not fit and R. cham. is on leaves.

But I wonder about the excipulum. Your photo shows only the cortex of very small globose cells. Are they larger inside?

Zotto

Nina Filippova, 10-06-2014 18:14
Re : Sclerotinia tetraspora
Hello, Zotto.

The species has distinct scletorium (not stromatized tissues or black stroma), which points to the genus Sclerotinia.

I have seen Rutstroemia chamaemori on this plant (likely, but spores were curved which is not mentioned in descriptions) and showed it here before. http://www.ascofrance.com/search_forum/24922

Ciboria latipes - i have not met, but according to the key in Nordic Macromycetes (2000) it should have stromatized host tissues instead of a distinct sclerotium. Is it right?

I have microscopied excipulum once more, it is made from such small cells in upper part, no larger cells inside. Most inner tissue (medulla) made from interwoven hyphae. Cells become some larger at the base of receptacle. Probably the apothecia were also young, as many asci were underdeveloped.
Hans-Otto Baral, 10-06-2014 18:21
Hans-Otto Baral
Re : Sclerotinia tetraspora
Oh, I overlooked the small black sclerotium. Did you try a section of it to exclude that it contains some host tissue? This was formerly a basis to define Ciborinia.

The VBs in the paraphyses would point to a Botryotinia, by the way.