18-07-2025 23:03
Josep Torres
Hello.Fruitings between 51 and 130 microns in tota
16-07-2025 17:34
Bernard Declercq
Hello,I have trouble distinguishing above mention
14-07-2025 11:20
Michel Hairaud
Bonjour, Voici une espèce de (?) Hyaloscyphace
16-01-2023 21:31
Riet van Oosten
Hello, Nearby the find of Calycina claroflava on
14-07-2025 17:55
Yanick BOULANGERBonjourAutre dossier laissé en suspendJe viens de
14-07-2025 11:17
Yanick BOULANGERBonjourJ'ai un dossier Jackrogersella qui est rest
14-07-2025 15:52
Gernot FriebesHi,I wanted to share this collection on Rubus idae
14-07-2025 13:37
Gernot FriebesHi,do you think this collection could be R. ulmari
I found this Pyrenomycete, growing on wood of Acer campestre.
Perithecia size is 200-260um in diameter. Ostioles are 115-140um in diameter and have a cruciform shape.
Asci measured 55-68 × 5-7um, area spores 15-23 × 5-6.5um. With IKI, the apical apparatus becomes more apparent, but I'm not able to say if it is IKI +. in any case would be weakly positive.
Persistent paraphyses.
Following keys Rappaz, came to Eutypa Lejoplaca but I doubt if it could be another species, such as E. maura, or leptoplaca (if I consider the reaction to IKI is +).
Can anyone identify the species with the data provided?
Thank you,
Susana (Spain)
you are right, your fungus is likely around E. leptoplaca and E. lejoplaca. Eutypa maura can be ruled out because it is distinctive in having more immersed perithecia and the wood markedly blackened beneath the surface between the necks.
The most reliable difference between the two species is the reaction of the apical apparatus to iodine.
I suggest you follow Rappaz's procedure with a short pretreatment of perithecial content in 3% KOH and mounting in Melzer's reagent. This leads to a more drastic retraction of the ascal content than IKI and makes the apical apparatus easier to make out. Remember it is in fact subapical, at the bottom of a shallow tubular structure. The thing is to work on a very small amount of material in order to make a very thin slide, otherwise interpreting the iodine reaction is most often tricky, especially in species where the apical apparatus is very small. This applies to all species of Diatrypaceae since the character of reaction to iodine is crucial.
Come back with a clear result, whatever how long it takes!
Cheers,
Jacques
¿C'est la réaction tout aussi intense avec KOH + Melzer, qui KOH + IKI?
J'ai fait quelques préparations KOH + IKI (je n'ai pas de Melzer) et pense que c'est positif, j'observe dans les asques un couple de très petits points que je dirais sont bleus.
Que pensez-vous?
Saludos
Susana
ton excellente photo montre bien une réaction positive, amyloide. Tu peux donc écarter lejoplaca.
E. leptoplaca est beaucoup plus fréquente sur Fraxinus, tu devrais la trouver facilement sur des petites branches tombées récemment avec le vent et tu pourras ainsi lui comparer ta récolte.
Amitiés,
Jacques
Saludos.
Susana