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ITS sequences from the following two collections B

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Many spored Pyreno on Alnus
Stefan Blaser, 21-03-2012 12:47
Hello everybody

I could need some help with this Pyreno on most probably Alnus wood.


Description: Perithecia immersed below the Cortex, only visible as small bumps on the surface, sometimes pore just visible as Cortex cracks open. Perithecia with an ovoid lower part of about 0.5-1 mm in width and a height of about 0.5 mm. The upper part is disc like or semiglobous, consisting of dense black tissue with a pore in the middle. For the photo I removed the Cortex


Microscopy: The most interesting feature is the Ascus with many Spores. It's more than 20 spores  and I counted about 25 to 35 but counting was very difficult. I think it's 32- spored. The Apical apparatus is very well visible and coloured in Kongo and roundish (picture) but doesn't react in Iodine.  I'm not sure whether the Spores are mature and I guess probably not. Spores were completely hyaline, nonseptate and 20-24 x 3.5-4.5 micrometers  with blunt appendixes of about 1-2 micrometers of length. These appendixes were only observed in water (still difficult to see!) and  could not be detected in KOH, Kongo  or Iodine. Sometimes there seemed to be a slime sheath around the Spores, however this was  only observed very infrequently.


Thank you for any help


Stefan


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Björn Wergen, 21-03-2012 13:30
Björn Wergen
Re : Many spored Pyreno on Alnus
Hi Stefan,

it could be Ditopella ditopa. If you want, send me, I am interested in it (it seems as if you are good in finding rare pyrenos :D ).

regards,
björn
Stefan Blaser, 21-03-2012 14:47
Re : Many spored Pyreno on Alnus
Hi Björn

I came by Ditopella when looking in the Ellis book. However I have more than 16 spored Asci (constantly) and never seen a septate Spore (which could be because of immature Spores) and appendages as I've seen them were also not described in Ellis. Do you have another description of Ditopella which includes those features?

Danke und lieber Gruss
Stefan

Björn Wergen, 21-03-2012 18:44
Björn Wergen
Re : Many spored Pyreno on Alnus
Hi Stefan, Hi Yan,

yes, I also think it should be the Ditopella. I have a description in BARR 1978: "Asci 16 to 24 spored" and ROSSMAN 2007, who describes D. ditopa as an 8-spored species. 

Species with a variability in number of spores in Asci are not rare in Diaporthales (see Valsa nivea, for example). I think your finding is imature and does not show septated spores ;)

regards,
björn

PS: if you wish to have something about Diaporthales, tell me.
Björn Wergen, 21-03-2012 21:18
Björn Wergen
Re : Many spored Pyreno on Alnus
I can. I have seen Valsa nivea-asci with 8, 4 and even only 3 spores in one collection. That was what I have meant by variability, and I thought that it could be similar with the Ditopella.

regards,
björn
Stefan Blaser, 21-03-2012 21:28
Re : Many spored Pyreno on Alnus
Hi Yan and Björn

Many thanks for the help. I think the identification is settled.

bye
Stefan
Hiran Hiran Anjana Ariyawansa, 27-03-2012 16:15
Re : Many spored Pyreno on Alnus
I would like to go through this specimens it seems to be a quite interesting fungi
hmm better to do  sequencing i think
Can you send me a peace of specimens
Thanks
Hiran
Björn Wergen, 27-03-2012 16:17
Björn Wergen
Re : Many spored Pyreno on Alnus
Of course I will send it to you in the next package, Hiran ;)
Hiran Hiran Anjana Ariyawansa, 27-03-2012 16:24
Re : Many spored Pyreno on Alnus
Thanks a lot hope to see it sooon Thanks KIT