28-08-2022 11:04
Malcolm Greaves
I use this for staining the warts on Scutellinia a
24-08-2022 17:24
Alex Akulov
Dear colleagues,I am asking for your help in ident
24-08-2022 07:24
Lothar Krieglsteiner
.. found yesterday in the Eifel National Park, Ger
23-08-2022 19:48
Stephen Martin Mifsud
I have found this wonderful species on Phoenix cf
23-08-2022 20:41
Marcus YeoThis small discomycete was growing on dead leaves
22-08-2022 21:15
Lothar Krieglsteiner
Hello,does somebody have this for me:Sclerotiniace
21-08-2022 12:21
Joeri Beliswhite fruitbodies on dead leave of Viburnum tinus
20-08-2022 15:49
Georges GreiffDear all,I would be most grateful for the followin
Lactophenol Cotton Blue
Malcolm Greaves,
28-08-2022 11:04
I use this for staining the warts on Scutellinia and also when heating spores to see if the outer surface loosens. I have just spotted that one bottle I have is LCB but another is just Cotton Blue in Lactic Acid with no mention of the Phenol. Does anyone know what component of the stain causes the loosening?Thanks
Mal
Viktorie Halasu,
28-08-2022 11:44
Re : Lactophenol Cotton Blue
Hi Mal,
I usually use small amount of LACB to not overstain the preparate. When I suspected a species with loosening perisp. but it didn't show it even after gentle heating, I added a drop of lactic acid and then it loosened all right (after more heating or without, I don't remember). Pure LA is very viscous and goes rather slowly into the solution, I usually have to lift the edge of the coverslip a bit to help it overflow the section. And S. mirabilis preparate was not heated at all, but I left it overnight and the perisp. loosened too. I don't have any exper. with phenol, though.
Have a nice sunday.
Viktorie
I usually use small amount of LACB to not overstain the preparate. When I suspected a species with loosening perisp. but it didn't show it even after gentle heating, I added a drop of lactic acid and then it loosened all right (after more heating or without, I don't remember). Pure LA is very viscous and goes rather slowly into the solution, I usually have to lift the edge of the coverslip a bit to help it overflow the section. And S. mirabilis preparate was not heated at all, but I left it overnight and the perisp. loosened too. I don't have any exper. with phenol, though.
Have a nice sunday.
Viktorie
Malcolm Greaves,
28-08-2022 22:36
Re : Lactophenol Cotton Blue
Thanks Viktorie