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30-10-2014 22:05

Garcia Susana

Hi all: I collected these apothecia of grayish co

04-11-2014 14:47

Castillo Joseba Castillo Joseba

En madera sumergida en aguaPuede ser  Cudoniella

04-11-2014 16:19

Castillo Joseba Castillo Joseba

de 3,3 - 0,5 mm en acaciano se por donde mirar

04-11-2014 16:18

Castillo Joseba Castillo Joseba

de 3,3 - 0,5 mm en acaciano se por donde mirar

04-11-2014 12:25

Blasco Rafael Blasco Rafael

Hola, Tener el siguiente articulo Alguien Floreria

11-10-2014 09:04

Blasco Rafael Blasco Rafael

Hola, buenos días, les parece que la Muestra sea

03-11-2014 20:43

Spooren Marco Spooren Marco

Hello Stip, thanks for the Keissler, Flechtenparas

03-11-2014 15:11

Mafalda Freire Fernando Mafalda Freire Fernando

Could you help me with this one?Samson, R.A. &

31-10-2014 19:46

Ralph Vandiest Ralph Vandiest

Hello, I found this species along a road in a coa

01-11-2014 22:56

Yannick Mourgues Yannick Mourgues

... j'ai trouvé ce truc-là que je ne sais où ch

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Mollisia humidicola?
Garcia Susana, 30-10-2014 22:05
Hi all:

I collected these apothecia of grayish color in the hymenium and dark brown in excipulo.
The reaction with KOH is negative.
Asci are IKI + (blue). 68-75x6.5-7.5um size. Croziers +
Spores with a septum (difficult to distinguish in water, but visible in IKI).
Given the features mentioned, I think it may be mollisia humidicola. Can anyone confirm or refute the determination?


Thanks in advance, greetings
Susana

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Hans-Otto Baral, 30-10-2014 22:53
Hans-Otto Baral
Re : Mollisia humidicola?
Yes, clarly humidicola, or M. luctuosa as I believe is the older name.

You have also nonseptate spores on your photos, those in which an "empty" space is in the middle (the nuclear region). When the oil drops are found in the centre then there is also a septum.

Tell me please what was the substrate, indet. grass?
Peter Püwert, 31-10-2014 01:08
Peter Püwert
Re : Mollisia humidicola?
Hi all,
Mollisia humidicola/ luctuosa is in our distrikt a frequently species on grasses. This species has been found on
Calamagrostis epigejos, Carex spec., Carex rostrata, Juncus effusus, Molinia coerulea, Phalaris arundinacea, Scirpus sylvaticus.
Greetings
Peter Püwert.
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Garcia Susana, 31-10-2014 08:13
Re : Mollisia humidicola?
Hi,
thanks

I do not know what type of substrate is, but it is not a grass. It is a piece of hardwood branch, collected in an area where they grow elms.


Saludos


Susana

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Hans-Otto Baral, 31-10-2014 09:16
Hans-Otto Baral
Re : Mollisia humidicola?
Dear Susana

could you please try to make a cross cut of the substrate where it is not too rotten, i.e., where a section is still possible?  It must not be a microscopic section, but only a surface view on the wood. Wood is rather easily distinguished from herbs/monocots.

It would be a great surprize to see a species with siuch long, septate spores on ligenous substrate. The diameter of the piece seems to lie at about 15-20 mm, isn't it? There is no large grass with such thick base?

Zotto
Martin Bemmann, 31-10-2014 09:47
Martin Bemmann
Re : Mollisia humidicola?
possibly a stem base of a larger umbellifer. Should be hollow then.

Regards
Martin
Garcia Susana, 31-10-2014 14:14
Re : Mollisia humidicola?
As I can I will try to do a cross-section of the substrate

Saludos
Garcia Susana, 31-10-2014 19:47
Re : Mollisia humidicola?
Hello again

This is a cross section of the substrate.


 


Un saludo


Susana

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Hans-Otto Baral, 31-10-2014 20:24
Hans-Otto Baral
Re : Mollisia humidicola?
Thanks, this is very useful. It looks like wood, I don't think that any monocot has such an anatomy, although scattered vascular bundles are typical there.

I assume the substrate is rather hard, is it? This section covers only about 2 mm of the centre, does it? How thick is the entire stem?

Ulmus seems completely excluded. Salix could be a possibility, but I would expect a parenchymatic inner region that I do not see here.

I do not want to bother you, but now that you got such a fine cross section, a radial section would be useful to see the pore perforations and the pits.

Zotto
Garcia Susana, 31-10-2014 21:39
Re : Mollisia humidicola?
It is not a bother.

Yes, the substrate is hard.
Yes, the section is about 2mm
Entire stem is 15mm, but it is very deteriored.


I send you a pictures from longitudinal cut. I hope the pictures will help you. I do not know what needs to look


Susana

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Hans-Otto Baral, 31-10-2014 22:46
Hans-Otto Baral
Re : Mollisia humidicola?
The anatomy looks really like wood, but now I see the section you made was through a root. I did not closely look so far at your survey photo, and Martin drew my attention to this. Indeed, it looks more like a herb, among which also rather woody exist, and I do not believe a tree like Salix has such roots.

Helpful would be to have a section through the 15 mm thick part. I assume this is difficult, but it is enough to know whether the interior is hollow, and how thick the wall. The surface looks rilled, does it?
Garcia Susana, 03-11-2014 07:55
Re : Mollisia humidicola?
Hello Zotto,

You're right. Effectively, the interior is hollow and the surface is rilled. The wall thickness is about 3.5mm. I send you a photo of the cut.


a greeting
Susana

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Hans-Otto Baral, 04-11-2014 11:32
Hans-Otto Baral
Re : Mollisia humidicola?
I got a new idea: You did not show us living paraphyses, did they contain the obligatory elongate VBs typical of Mollisia? Your fungus could actually be a Pyrenopeziza, P. chailletii/carduorum. I am almost sure, also the macros I have fit well.

That would fit to the substrate!

Zotto
Garcia Susana, 04-11-2014 12:19
Re : Mollisia humidicola?
And the septate spores?

Susana
Hans-Otto Baral, 04-11-2014 12:32
Hans-Otto Baral
Re : Mollisia humidicola?
Well, I think your fungus develops septate spores only at overmature stages. That can be found out only when having living asci. Inside them I suppose they are non-septate.
Garcia Susana, 04-11-2014 12:37
Re : Mollisia humidicola?
But, in picture nº 3, are septate spores inside asci.
Hans-Otto Baral, 04-11-2014 12:45
Hans-Otto Baral
Re : Mollisia humidicola?
They are dead asci, therefore it says nothing. This is a very frequent misbelief to think that spores outside asci must be more mature than inside. True is it only when you compare spores inside living asci with those being actively ejected, and even then they can have just the very same grade of maturity, if they were freshly ejected.

Within dead asci the spores often remain alive and finally develop a septum or become brown etc.

You did not make a water preparation of the fresh fungus without applying much pressure? Then we could also see the living paraphyses.

Garcia Susana, 04-11-2014 20:23
Re : Mollisia humidicola?
I made a new preparation in water.
Effectively, the paraphyses are not mollisia type.
I see septate spores inside the ascus.
What do you think?
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Hans-Otto Baral, 04-11-2014 20:49
Hans-Otto Baral
Re : Mollisia humidicola?
Your second photo shows a living paraphysis with two guttules in the middle - these are SCBs and typicla of Pyrenopeziza.

The asci are completely dead. Living asci look very different , much wider. If you had dried the sample then no wonder when you find mainly dead elements. Asci are most sensitive to drying.
Garcia Susana, 04-11-2014 21:37
Re : Mollisia humidicola?
Thanks

Saludos
Susana