18-03-2020 19:03
Suhaib Firdous Yatoo
What is the best way to preserve Ascobolaceae memb
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Preservation of Ascobolaceae members
Suhaib Firdous Yatoo,
18-03-2020 19:03
What is the best way to preserve Ascobolaceae members, mostly the smaller ones that are less than 1mm and how to preserve them for herbarium purposes?How to rehydrate if I want to work on them again?
(as KOH dissolves the episporium).
Hans-Otto Baral,
18-03-2020 19:05
Re : Preservation of Ascobolaceae members
The by far very best way is to study the in the fresh state. Everything else leads to minor results and includes artifacts.
Suhaib Firdous Yatoo,
19-03-2020 10:49
Re : Preservation of Ascobolaceae members
How to preserve them for herbarium?
And can we preserve the smaller ones as slides mounted in Hoyer?
And can we preserve the smaller ones as slides mounted in Hoyer?
Hans-Otto Baral,
19-03-2020 10:55
Re : Preservation of Ascobolaceae members
I can only recomend you to read my paper from 1992 (Mycotaxon).
Preservation in Ascobolus is not different from any other ascomycete. Drying and placing in a tight plastic bag. Hoyer's liquid I remember a bit, it is killing the cells and perhaps also dissolves structures.
Suhaib Firdous Yatoo,
19-03-2020 11:07
Re : Preservation of Ascobolaceae members
Yes, and cells seem to swell a bit.
Thank you very very much sir.
Thank you very very much sir.